pET SUMO Protein Expression System

The pET SUMO vector allows expression of a recombinant protein with an. N-terminal peptide containing the 6xHis tag and SUMO fusion protein. General guidelines ...

由 MS Team 著作 — Have you ever thought of using a solubility tag to enhance expression of your protein, considered using SUMO and then wondered, which sequence should I use?

由 D Kuo 著作 · 2014 · 被引用 45 次 — A solution to this problem is the expression of proteins as fusions to solubility tags such as the SUMO protein. SUMO fusion proteins can be cleaved to remove ...

SUMO-tag Protein Expression System developed by Creative BioMart utilizes SUMO to allow expression, purification, and generation of native proteins in both ...

Highly specific and active protease for efficient cleavage of SUMO tags from proteins of interest; Contains N-terminal polyhistidine tag; Useful for further ...

由 TR Butt 著作 · 2005 · 被引用 634 次 — Fusing a tag to the target protein provides a one-step purification procedure by passing cell extracts or supernatants over an appropriate affinity matrix.

Sumo tag is most frequentely used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein.

Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein.

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to ...