Nuclease P1 Digestion for Bottom

由 C Romier 著作 · 1998 · 被引用 144 次 — Recognition of single-stranded DNA by nuclease P1: high resolution crystal structures of complexes with substrate analogs. Proteins. 1998 Sep 1;32(4):414-24.

由 JD Jones 著作 · 2023 · 被引用 6 次 — Nuclease P1 is an endonuclease with no nucleobase specificity that is commonly used in a two-step enzymatic process to digest RNA down to ...

Nuclease P1 from Penicillium citrinum lyophilized powder, >=200 units/mg protein (E1%/280, 3-5-Phosphodiesterase). NT$35,900. 品牌:SIGMA 品號:N8630

Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food ...

由 M Silberklang 著作 · 1977 · 被引用 276 次 — The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial ...

Nuclease P1 is a zinc-dependent single-strand specific nuclease which hydrolyzes 3'->5' phosphodiester bonds in RNA and ssDNA.

核酸酶P1 是一种锌依赖的单链特异性核酸酶，可水解RNA 和单链DNA（ssDNA）中的3´→5´ 磷酸二酯键。

One unit of nuclease P1 is added per 24 mg total nucleic acid. Digestion is carried out at pH 8.0,55°, for 2 hr, and the nucleic acids are again denatured and ...

Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts. The enzyme has an optimal temperature of approximately ...