Nuclease protection assay

由 MF Carey 著作 · 2013 · 被引用 26 次 — These nucleases digest the single-stranded overhang regions of RNA molecules, but RNA–RNA hybrids are resistant to cleavage. This resistance forms the ...

由 M Goldrick 著作 · 2003 · 被引用 10 次 — Nuclease protection assays (S1 nuclease protection and RNase protection) are extremely sensitive procedures for detection and quantitation of mRNA species ...

由 YJ Ma 著作 · 1996 · 被引用 63 次 — The RNase protection assay is a highly sensitive technique developed to detect and measure the abundance of specific mRNAs in samples of total cellular RNA.

Nuclease protection assays are a highly sensitive, solution-based technique used to detect and quantify specific RNA targets from complex RNA mixtures. From: ...

NPAs are the method of choice for the simultaneous detection of several RNA species. During solution hybridization and subsequent analysis, individual probe/ ...

The RNase Protection Assay (RPA) is a widely used technique for the detection and quantitation of mRNA.

Nuclease protection assays are used to map 內含子s and 5' and 3' ends of transcribed 基因 regions. Quantitative results can be obtained regarding the amount of ...