RNAi選擇指南

We explore the differences between the two methods for ablating gene function, and situations where one technology is more appropriate. Knockout by TALEN or ...

由 MA McAnuff 著作 · 2007 · 被引用 108 次 — Analysis of the data established that shRNA was significantly more potent than siRNA at mediating knockdown when compared on a mole basis. The combination of ...

shRNA molecules are processed within the cell to form siRNA which in turn knock down gene expression. The benefit of shRNA is that they can be incorporated into ...

RNAi可以分為外源性與內源性2種，外源性有siRNA/shRNA與內源性的miRNA: siRNA(small interfering RNA):. 主要都是外來的雙股RNA分子，藉由轉染(Transfection)或電穿孔 ...

2014年10月23日 — RNA interference (RNAi) uses short double-stranded RNAs (dsRNAs) to selectively knock down gene expression. Short interfering RNA (siRNA), ...

由 DD Rao 著作 · 2009 · 被引用 858 次 — ... knockdown approaches. Methods of mediating the RNAi effect involve small interfering RNA (siRNA), short hairpin RNA (shRNA) and bi ...

2024年1月12日 — siRNA is unstable and largely depends on an effective in vivo delivery system. In contrast, shRNA is superior to siRNA in knockdown genes and ...

这篇综述将重点介绍shRNA和siRNA是如何导致蛋白质表达抑制的。通过几种蛋白的活性（下面讨论），通过短反义核酸（siRNA和shRNA序列）锁定细胞mRNA，从而实现其随后 ...